The Diagnosis of Sheep Cystic Echinococcosis by Native Antigen B - Elisa Method

Cystic echinococcosis (CE) is one of the most prevalent zoonotic diseases in some countries world. considered a neglected disease. This disease increases economic damage via medical costs and loss human livestock productivity. The aim current study gains better understanding prevalence CE sheep. Totally 250 sheep sera were collected. 25 serum samples from newborn lambs used as negative, others obtained slaughterhouses positive to infection with 200 unknown sera. Antigen B Isolated hydatid cysts fluid was for designing ELISA methods. first Method anti-Sheep conjugate (SIGMA, at 1:3000 dilution). According results, seroprevalence East Azerbaijan Iran 5.5% sensitivity specificity diagnosis hydatidosis by AgB-ELISA methods 92%. Using design has attracted researchers recent years. During this study, an Iranian native antigen specific detection using technique. results have shown that so valuable.